Dynamic Changes of Polyphenol Contents and Enzyme Relating to Polyphenol Synthesis in Sun-cured Tobacco in Jilin
-
摘要: 选取吉林当地3个主栽晒烟品种研究了不同晒烟品种叶片发育过程中多酚类物质及其相关酶活性的动态变化以及两者之间的关系.结果表明,不同晒烟品种中总酚含量表现为青黄叶 > 大叶黄 > 大白花,总酚在整个生育期的含量为0.16%~1.2%.不同晒烟品种中总酚含量随着叶龄的增加而提高,在成熟期稍有降低;青黄叶和大叶黄中绿原酸、芸香苷和莨菪亭在叶片发育过程中整体上表现出上升趋势,而大白花中绿原酸、芸香苷和莨菪亭含量呈单峰曲线.各品种中苯丙氨酸解氨酶活性与绿原酸和芸香苷的变化规律相同,多酚氧化酶活性和过氧化物酶活性呈单峰趋势.在烟叶生长过程中,各晒烟品种中苯丙氨酸解氨酶活性与绿原酸、芸香苷、莨菪亭和总酚的变化呈正相关,且与绿原酸和芸香苷达到显著水平;多酚氧化酶活性变化与绿原酸、芸香苷、莨菪葶和总酚的相关性表现为负相关,但未达到显著水平;过氧化物酶活性与绿原酸、芸香苷、莨菪葶和总酚呈正相关,且与总酚达显著水平.由此可见,苯丙氨酸解氨酶和过氧化物酶与多酚类物质之间有显著的相关性,可以通过调节这两种酶的活性来控制多酚的含量,从而改善烟叶质量.Abstract: A field experiment was conducted to study the changes of polyphenol contents and enzyme relating to polyphenol synthesis and the relationship between them in three sun-cured tobacco varieties in Jilin. The results showed that the total phenols content Qinghuangye > Dayehuang > Dabaiihua in the three sun-cured tobacco varieties in Jilin, with range 0.16% to 1.2% in the whole growth period. The total phenols increased with increasing leaf age, and decreased slight in late mature. Chlorogenic acid, rutin and scopoletin showed an upward trend during leaf development in Qinghuangye and Dayehuang, but they showed a single peak curve in Dabaihua. Phenylalanine ammonia lyase (PAL) activity had the same trend with chlorogenic acid and rutin in all varieties. Polyphenol oxidase (PPO) activity and peroxidase (POD) activity showed a single peak trend. With the growth and development of tobacco leaves, phenylalanine ammonia lyase activity with chlorogenic acid, rutin, scopoletin changes and total phenols were positively correlated, and with chlorogenic acid and rutin was significantly correlated. Peroxidase activity with chlorogenic acid, rutin, scopoletin and total phenols were positively correlated, and with total phenol reached significant levels. There was significant correlation between phenylalanine ammonia lyase, peroxidase and polyphenol contents. We can regulate the activity of two enzymes to control content of polyphenols, then improving the quality of tobacco finally.
-
Keywords:
- polyphenol contents /
- phenylalanine ammonia lyase /
- polyphenol oxidase /
- peroxidase
-
-
[1] Sook M E. 凝胶色谱应用于卷烟烟气酚类物质的特性鉴定[J]. 烟草科技,1983(1):58-59. [2] 朱小茜,徐晓燕,黄义德,等. 多酚类物质对烟草品质的影响[J]. 安徽农业科学,2005,33(8):1910-1911. [3] 朱大恒. 烤烟发酵过程中品质及香吃味的形成[J]. 河南农业科学,1997(3):5-7. [4] 钟庆辉. 烟草芳香吃味化学指标的探索[J]. 烟草科技,1981(4):21-23. [5] 武维华. 植物生理学[M]. 北京:科学出版社,2003. [6] 王瑞新. 烟草化学[M]. 北京:中国农业出版社,2003. [7] Penn P T, Stephens R L, Weybrew J A. Some factors affecting the content of principal polyphones in tobacco leaves [J]. Tab. Sci., 1958(2): 68-72..
[8] 宫长荣,王爱华,王松峰. 烟叶烘烤过程中多酚类物质的变化及与化学成分的相关分析[J]. 中国农业科学,2005,38(11):2316-2320.. [9] 过伟民,张骏,刘阳,等. 烤烟质体色素及多酚与外观质量关系研究[J]. 中国烟草学报,2009,15(2):33-40. [10] 席元肖,宋纪真,杨军,等. 不同烤烟品种的类胡萝卜素、多酚含量及感官品质的比较[J]. 烟草科技,2011(2):70-74. [11] 过伟民,蔡宪杰,魏春阳,等. 豫中浓香型烤烟感官质量与部分质量指标的关系[J]. 烟草科技,2010(6):22-27. [12] 国家烟草专卖局. YC/T202—2006 烟草及烟草制品多酚类化合物绿原酸、莨菪亭和芸香苷的测定[S]. 北京:中国标准出版社,2006. [13] 中国科学院上海植物生理研究所. 现代植物生理学实验指[M]. 北京:科学出版社,1999. [14] 白宝璋. 植物生理学测试技术[M]. 北京:中国科学技术出版社,1993:98-106. [15] 张志良. 植物生理学实验指导[M].北京:北京高等教育出版社,1993:136-142. [16] 徐晓燕,孙五三,王能如. 烟草多酚类化合物的合成与烟叶品质的关系[J]. 中国烟草科学,2003,24(1):3-5. [17] 俞京,章平泉,龚珍林,等. 烤烟主要多酚与其感官质量的典型相关性[J]. 烟草科技,2010(1):38-43. [18] 宗浩,扬程,陈刚,等. 不同烤烟品种香型风格与多酚类物质含量差异分析[J]. 中国农学通报,2011,27(30):241-245. [19] Amrita Kumar, Brian E Ellis. The phenylalanine ammonia-lyase gene family in raspberry. Structure, expression, and evolution [J]. Plant Physiology, 2001, 127(1): 230-239.
[20] 戴亚, 施春华. 烟草多酚氧化酶的分离提纯及性质研究[J]. 重庆烟草,2002(2):29-32. [21] 郭红莲,程根武,陈捷,等. 玉米灰斑病抗性反应中酚类物质代谢作用的研究[J]. 植物病理学报,2003,33(4):342-346. [22] 王爱华,王松峰,宫长荣. 氮素用量对烤烟上部叶片多酚类物质动态的影响[J]. 西北农林科技大学学报,2005,33(3):57-60. [23] 马俊峰. 硫酸铜对烟叶生长过程中多酚和色素的调控效应及影响[D]. 郑州:河南农业大学,2009.
计量
- 文章访问数: 262
- HTML全文浏览量: 0
- PDF下载量: 206
下载:
