UPLC-MS/MS法测定烟草中12种常用农药残留量

Determination of Twelve Common Pesticides Residues in Tobacco by Using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

摘要: 为了实现烟草中常用农药残留量的快速筛查,建立了超高效液相色谱-串联质谱(UPLC-MS/MS)快速分析烟草中12种农药残留量的方法,烟叶样品采用乙腈提取、PSA净化和UPLC-MS/MS分析测定。12种农药在低、中、高(2、200、500 µg/kg)3个加标水平的回收率范围为62.1%~126.3%,相对标准偏差(RSD)为0.6%~14.8%,检出限为0.24~4.24 µg/kg。本方法具有针对性强,检测时间短和灵敏度高的特点,能够很好的应用于烟草中常用农药残留量的快速分析检测。

Abstract: In order to rapidly determine common pesticide residues in tobacco, a rapid method for determining twelve pesticides in tobacco using UPLC-MS/MS was developed.Samples were extracted by acetonitrile, cleaned up by dispersive solid-phase extraction and finally detected by UPLC-MS/MS.The results showed that the recoveries of twelve pesticides ranged from 62.1%-126.3% at the spiking levels of 20 µg/kg, 200 µg/kg and 500 µg/kg.The RSD ranged from 0.6%-14.8%, and the LOD was from 0.24 µg/kg to 4.24 µg/kg.For the superior sensitivity, targeted residues and shorter analysis time, this method was suitable for the rapid analysis of common pesticide residues in batches of tobacco samples.