Abstract: Field experiment was conducted to explore the feasibility of reducing chemical fertilizer rate with bio-organic fertilizer applied on tobacco production and its proper reducing proportion in Dali.The experiment included 5 treatments (0%, 15%, 30%, 45%, 60% chemical fertilizer rate reduced with equal quantities of bio-organic fertilizer).Yield, economic value, chemical component and sensory evaluation quality were measured.The results showed that the treatment of reducing 30% chemical fertilizer rate would not hinder the growth of flue-cured tobacco, and was beneficial to economic character, chemical composition, and sensory evaluation quality of tobacco leaves.The other treatments caused adverse effects in different extent on tobacco production.Therefore, it was feasible to reduce chemical fertilizer rate with bio-organic fertilizer applied on tobacco production in Dali, and the proper reducing proportion of chemical fertilizer rate is 30%.