Abstract: In order to explore the supporting technologies on fine seed and fine method of NC102 suitable for local natural conditions, and meet the need of industrial cigarette formula, effects of nitrogen rate on plants growth and yield of NC102 were studied by field experiment. The experiment set three replicates and four different nitrogen rate levels (60, 75, 90, 105 kg/ha). The results showed that nitrogen rate had significant effect on plant height, leaf area and yield. According to comprehensive analysis, higher field uniformity, lighter incidence of field diseases and higher production efficiency were recorded in the treatment of 75 and 90 kg/ha nitrogen rate, compared with the treatments of 60 and 105 kg/ha under moderate fertility (alkaline hydrolysis nitrogen 50-80 mg/kg). The highest average price and production efficiency were observed in the treatment of 75 kg/ha nitrogen rate. Based on chemical composition of flue cured tobacco leaves, when nitrogen rates were 75 and 90 kg/ha, sugar alkali ratios of the leaves were more harmonious, and they were accepted more easily by the cigarette industry. On the consideration of comprehensive analysis, the treatment of 75 kg/ha nitrogen rate was best on growth and yield of tobacco NC102.