Abstract: In order to explore the changes of Maillard reaction compounds during natural aging of flue-cured tobacco, GC-MS was used to determine content of Maillard reaction compounds in tobacco leaves from Zhucheng. The results were as follows: content of Maillard reaction compounds in upper leaves increased gradually in the first 18 months, and reached the highest value in the 18th month. The content then kept stable after that. The content of Maillard reaction compounds in middle leaves increased slowly in the first 30 months, and significantly increased to 52.44μg/g from the 30th to 36th months. Contents of 3-hydroxy-2-butanone, amyl alcohol, 2-pentyl furan, etc. increased during aging. Content of other chemical components had no significant change. Maillard reaction compounds were significantly correlated to aroma quality, aftertaste, mixed gas, irritation and total score in upper leaves, and indole, 2-methyltetrahydrofuran-3-ketone, nonanal, linalool oxide-1, furfuryl alcohol and other substances had a significant positive effect on smoking quality. On the other hand, 2,6-nonadienal and 2- pentyl furan had significant adverse effects on smoking quality in upper leaves. The simple correlation between Maillard reaction compounds and aroma concentration were significant at the level of 0.01 in middle leaves. In addition, aftertaste, mixed gas, irritation and total score were significantly affected by Millard reaction compounds. Indole, furfural, 2-acetylfuran,etc. had significant positive effects on smoking quality in middle leaves.In conclusion, aging can increase the content of Maillard reaction compounds and improve smoking quality of tobacco leaves.