Abstract: In order to improve fertilizer use efficiency and increase farmers' income as well as to protect the environment, field experiment was conducted to study effects of reducing fertilization on growth, yield and quality of flue-cured tobacco compared with the conventional fertilization in five different rotation systems (rape-tobacco, fallow-tobacco, green manure-tobacco, barley-tobacco and wheat-tobacco) in Xundian county of Yunnan Province The results showed that reducing fertilizer application had no adverse effect on growth and maturity of tobacco plants with rape, green manure, barley and wheat as preceding crops. Moreover, dry matter accumulation increased for the plants in the treatments with reducing fertilization after transplanting. Leaf yield, economic value, profit, chemical components and leaf quality were all better in the treatments with reduced fertilizer application than those with normal fertilization in four rotation systems, including rape-tobacco, green manure-tobacco, barley-tobacco and wheat-tobacco. However, for the tobacco plants in fallow-tobacco rotation system, reducing fertilization resulted in poor growth and undesirable quality. Leaf yield, economic value, profit, chemical components and leaf quality worsened. In conclusion, nitrogen fertilizer could be reduced by 25% and 9% for the tobacco plants compared with the normal fertilization rate in green manure-tobacco and rape-tobacco rotation systems, while for those in barley-tobacco and wheat-tobacco systems, potassium could be supplied at 90% of the recommended rate. For those in fallow-tobacco rotation system, nitrogen fertilizer should not be reduced by 17%. Further study is needed to determine the suitable nitrogen amount to be applied in this system.