Abstract: To evaluate the content of cembrenediol in various sun-cured cultivars, 11 sun-cured cultivars from 6 regions were analyzed by GC-MS. The results showed that: (1) the order of cembrenediol content in different cultivars of sun-cured tobacco was: Pipaye> Yangmei> Heiyan> Cunsanpi> Mayanghong> Xiaoliuye> K326> Erhei> Maer> K16> Heilao> Gonghui, and the 11 cultivars were clustered into three groups based on cembrenediol content; (2) the order of cembrenediol content in different types of sun-cured tobacco was: light red sun-cured tobacco > red sun-cured tobacco > dark red sun-cured tobacco > yellow sun-cured tobacco; (3) Except Maer, cembrenediol content in middle leaves was higher than that in upper leaves; (4) Correlation analysis showed that there was a linear correlation between α-2,7,11-cembtratriene-4,6-diol and β-2,7,11- cembtratriene-4,6-diol, with a correlation coefficient of 0.9576. The results from this study could be used in evaluating quality of different sun-cured tobacco cultivars.