Effect of Soil Moisture on Cyst Nematode Infection and Nicotine Content in Tobacco
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摘要: 为探究土壤水分对烟草孢囊线虫(Heterodera glycines)侵染的影响及其机制,2014年6—9月采用3种不同水分处理的盆栽试验,定期取样测定土壤和根系中线虫数量及根系和叶片中烟碱含量。结果表明,常规浇水处理下烟草孢囊线虫明显发生3代,繁殖系数(Rf=Pf/Pi)高达2.7;烟草根系中烟碱含量均低于1.00%。8月过度浇水处理下孢囊线虫第3代发生不明显且向后推迟,Rf为0.5;根系中最高烟碱含量(1.92%)发生在过度浇水15 d后。7—8月过度浇水处理下孢囊线虫第3代发生不明显,且第2代和第3代均向后推迟,Rf仅为0.1;根系中最高烟碱含量(3.22%)发生在过度浇水35 d后。常规浇水处理下,烟草叶片中烟碱含量总体呈上升趋势,而过度浇水处理下叶片中烟碱含量下降。过度浇水明显抑制烟草孢囊线虫的侵染,且与烟草根系中烟碱的大量积累可能存在相关性。Abstract: In order to explore the effect of soil water content on the infection of tobacco cyst nematode (Heterodera glycines) and the possible mechanism, pot experiments with three water treatments were carried out from June to September of 2014, the number of different-staged nematodes in soil and root and the nicotine content of tobacco leaf and root were determined. The results showed that, under normal water treatment, cyst nematode obviously occurred 3 generations, with a high reproduction factor (Rf=Pf/Pi) of 2.7, and the nicotine content in tobacco roots was lower than 1.00%. In the overwatering treatment in August, the third generation of cyst nematode barely occurred and was retarded, with the Rf of 0.5, and nicotine content in tobacco roots reached the maximum (1.92%) 15 d after the overwatering treatment. In the overwatering treatment in July and August, the third generation of cyst nematode barely occurred and both the second generation and the third one were retarded, with a small Rf of 0.1, and the maximum nicotine content (3.22%) in tobacco roots was reached 35 d after the overwatering treatment. The nicotine content of tobacco leaf showed an overall rising trend under normal water treatment and decreased in both overwatering treatments. Overwatering obviously inhibited the infection of tobacco cyst nematode, which was somehow correlated with the accumulation of nicotine in tobacco roots.
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Keywords:
- Nicotiana tobacum /
- Heterodera glycines /
- overwatering /
- nematode infection /
- nicotine
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