Analysis of Genetic Diversity and Population Structure of Germplasm Resources in Nicotiana tabacum
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摘要: 发掘优良基因，为改良烟草品种提供理论依据。采用ABI-3500xl遗传分析仪对87份烟草种质材料进行多态性检测。在87份烟草种质中，41对SSR引物共检测出241条多态性位点，平均为5.88个位点。遗传相似系数为0.47~0.91，PIC值为0.23~0.91，平均为0.63。群体结构分析表明，当K=2时，△K值最大，即87份烟草材料可划分为2个类群P1和P2，P1类群又可划分为5个亚类，P2类群可划分为2个亚类。烟草种质资源遗传多样性丰富，群体结构划分与种质类型不完全相关。Abstract: The population structure and genetic diversity of tobacco provide a theoretical basis for identifying elite genes and improving varieties by breeders. Genetic diversity and population structure of 87 tobacco materials were evaluated using 41 polymorphic SSR markers screened from a total of 80 SSR primer pairs. The amplified fragment length polymorphism was detected by The Applied Biosystems 3500 xl Genetic Analyzer. The results revealed that a sum of 241 polymorphism sites were found using the 41 SSR primer pairs, with an average of 5.88 sites per primer pair. The genetic similarity coefficient ranged from 0.47-0.91, and the PIC averaged 0.63, ranging from 0.23-0.91. Results from the clustering analysis based on a model-based method indicated that when K=2, the largest delta K value, namely 87 tobacco materials can be divided into two groups (P1 and P2, with the P1 group further divided into five classes and the P2 group further divided into two classes. The genetic diversity of tobacco germplasm was abundant and the division of population structure was not completely related to germplasm type.
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Keywords:
- tobacco /
- SSR fluorescent markers /
- genetic diversity /
- population structure
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