Abstract: In order to develop environment-friendly and safe methods for controlling stored tobacco insects, the effect of three non-chemical methods on the control of Lasioderma serricorne (Coleoptera:Anobiidae), an important stored tobacco insect, at different developmental stages, was investigated in laboratory. The results showed that all of the three tested non-chemical methods, including sealing, oxygen degradation by deoxidizer (ODD) and controlled atmospheres storage by purging nitrogen (CASPN), had great controlling effect on L. serricorne of different developmental stages. For the same exposure period, CASPN exhibited the strongest effect with 100% corrected mortality of L. serricorne of different developmental stages after 6 d of exposure. ODD also had a strong effect with more than 91% corrected mortality of different developmental stage L. serricorne after 6 d of exposure. Sealing had poor controlling effect. Therefore, rationally combination of the three non-chemical methods should have huge potential for controlling L. serricorne in practice.