Abstract: To provide theoretical basis for the characteristics of tobacco flavor, this study aims to investigate the effects of transplanting date, fertilization and variety on refreshing fragrance of flue-cured tobacco and to optimize cooperatively cultivation technology. We carried on a three-factor-orthogonal test including transplanting stage, nitrogen and varieties. The results suggested that the influence of transplanting date on refreshing fragrance was the largest, followed by variety and fertilization. The contribution rate of transplanting, fertilizer and varieties on refreshing fragrance of flue-cured tobacco were 44.4%, 25.7% and 29.9%, respectively according to the range analysis of orthogonal test. Changing date of transplanting makes the relationship between meteorological factors and flue-cured tobacco growth change. The style of refreshing fragrance was better manifested when transplanting-date was earlier. Conversely, the refreshing fragrance was delimited by delaying transplantation. There was a significant correlation between Fen flavor of flue-cured tobacco and precipitation and daily average temperature and daily temperature difference. We can use rainfall and daily average temperature after topping to judge the effects of transplanting date on tobacco flavor style. In the dual effects of varieties and climate, excessive application of nitrogen is not conducive to the expression of Fen flavor. Nitrogen amount should not exceed 105 kg/ha in this experimental condition. There were differences in the expression of the style of different varieties in different locations, for example, K326 > Yunyan87 > NC71 in E'shan, Yunyan87 > K326 > Honghuadajinyuan in Ning'er, K326 > Yunyan87 > Honghuadajinyuan in Huaning. In conclusion, growers in different regions can choose tobacco varieties, do early transplanting appropriately, and lower fertilization to improve the flavor characteristics.