Abstract: A quantitative method using ultra performance liquid chromatography (UPLC) with UV detector was developed for the determination of α and β-2,7,11-Cembratriene-4,6-diol of tobacco.Ethyl acetate was used as solvent for extraction with a liquid-material ratio of 50:1.The ultrasonic frequency used was 45 kHz andthe temperature of extraction was room temperature above 25℃.The time of extraction was 10 min.The extracts obtained with above experiment conditions were dried by nitrogen and dissolved with mobile phases and the target compounds were separated on an ACQUITY UPLC BEH C18 column using acetonitrile-water (70:30) as mobile phase by gradient elution.The flow rate was set at 0.3 mL/min,the column temperature was 35℃ and quantitative wavelength of UV detector was set at 200 nm.The results indicated that the calibration curves of α and β-2,7,11-Cembratriene-4,6-dio were linear in the range of 12.14-194.2 μg/mLand 6.229-99.67 μg/mL respectively.The limits of detection (LOD,S/N=3) were 0.1166 and 0.1495 μg/mL respectively,and the limits of quantification (LOQ,S/N=10) were 33.8 μg/g and 49.8 μg/g respectively.The average recoveries of standard addition of samples were 96.7%-98.8% and 96.2%-98.4% respectively at three spiked concentration levels,and the relative standard deviations (RSDs) of reproducibility of the method were 3.46% and 3.11% respectively.There was no significant difference in the measurement results of tobacco samples between this method and the tobacco industry-standard method.The method was simple,sensitive and accurate,and was suitable for large scale determination of α and β-2,7,11-Cembratriene-4,6-dio in tobacco.