Abstract: Yuyan13, a new variety of flue-cured tobacco developed by crossing Zhongyan101 as female parent and 664-01 as male parent through hybridization and pedigree selection. Yuyan13 passed tobacco variety evaluation of China in April, 2016. Its genetic traits were stable with uniform populations. Yuyan13 grew vigoursly in fields, was tolerant to drought and easy to cure. Yuyan13 showed resistance to black shank, medium resistance to brown spot and root knot and moderate susceptibility to bacterial wilt, TMV, CMV and PVY. General disease resistance of Yuyan13 was better than NC89. The production and output value of Yuyan13 were high and the main economic traits were superior to the control cultivar NC89. Its cured leaf was rich in oil with well-distributed orange color. The chemical composition was favorable and well proportioned to meet the requirements of manufacturing cigarettes with Chinese characteristics. In conclusion, Yuyan13 is avariety which has better quality, resistance, stable yield, wide adaptability, which is suitable for planting in the Huanghuai area and part of the northeast region of China.