Abstract: Soil acidification is getting worse under long-term fertilization. In this study, a field experiment was conducted to investigate the effects of different soil conditioners on soil pH and tobacco growth in Fujian Province. No conditioner, local countermeasure (dolomite powder) were considered as controls. Multi-functional soil conditioners ST and MT were applied with half and the same amount as dolomite powder. The results showed that compared to the treatment of no conditioner, the conditioners significantly increased rhizosphere soil pH by 0.5-1.5 units, increased the ratio of high quality tobacco leaves by 1.5%-12.9%, and significantly increased the yield and output value by 4.0%-9.0% and by 6.0%-14.7%, respectively. Compared to dolomite powder, under the same applied amount, the treatments of multi-functional soil conditioners MT significantly increased the ratio for high quality tobacco leaves and the economic benefit by 11.2% and by 1485.7 Yuan/ha, respectively. Additionally, multi-functional soil conditioners applied with half amount of dolomite powder showed the equivalent conditioning effects with dolomite powder. These results suggested that the multi-functional soil conditioners have positive effects on soil pH and tobacco growth, thus, could be recommended to be used in tobacco production.