Abstract: In order to provide scientific guidance for rational application of micro-fertilizers in tobacco areas, it is necessary to study the degree of soil trace element abundance and its influencing factors. Based on 227 sample data, the spatial distribution characteristics and influencing factors of 5 trace elements, Fe, Mn, Cu, Zn and Mo, were analyzed by correlation analysis, regression analysis and Geostatistics methods. The results showed that 19.6% of the samples of soil from the studied areas were Mo deficient or extremely deficient, and the remaining 4 elements were at rich or extremely rich levels. The ratio of nugget effect was between 22.4%-50.0%, with strong or moderate spatial autocorrelation. The available molybdenum in soil showed a trend of high in northwest and low in southeast, and the other four trace elements showed a pattern of low in northwest and high in southeast. Organic matter, CEC, available nitrogen and available phosphorus and potassium had stronger effects on the spatial variability of the five elements, and soil type and elevation had a weaker influence. The previous crop had a significant effect on iron, manganese, copper and molybdenum content. Therefore, the researched areas should combine the spatial distribution characteristics of each element and the previous crop, reduce fertilizer input of effective iron, manganese, copper and zinc and other trace elements, and at the same time increase the amount of molybdenum fertilizer.