Abstract: In order to improve the imbalance of soil nutrients and the decline in quality of tobacco leaves caused by continuous cropping of tobacco in Huili County, the effects of different well-rotted farmyard manure application rates on tobacco quality and soil nutrients were studied. The results showed that the contents of available nitrogen, potassium and phosphorus in soils treated with farm manure were higher than those in CK. Well-rotted farmyard manure could increase the content of humic acid and fulvic acid in soil. 110 d after transplanting, the content of humic acid was up to 4.34 g/kg, which was 36.61% higher than that of CK, and the difference reached a significant level. HA/FA is also up to 0.73. The well-rotted farmyard manure could significantly reduce the content of sugars and increase the ratio of sugar to alkali and the ratio of potassium to chlorine. Compared with CK, the ratio of sugar and alkali decreased by 23.85% and 24.34% in C2F and B2F under 7500 kg/ha well-rotted farmyard manure, while the ratio of potassium to chlorine increased by 1.67 and 3.34. For C2F and B2F, the total amount of neutral substances treated by 7500 kg/ha well-rotted farmyard manure was also the highest, reaching 1163.50 and 683.24 μg/g, which was 15.71% and 60.00% higher than CK. A significant effect on improving the chemical composition coordination and increasing the aroma content of flue-cured tobacco was observed in the treatment of 7500 kg/hawell-rotted farmyard manure.