Abstract: In order to explore the difference and trends of the main physiological changes in different sections of tobacco leaves during curing, tobacco leaves with midrib removed were divided into three sections:leaf base (T1), middle leaf (T2), and leaf tip (T3). After that, they were cured with the whole leaf (CK) in the same temperature and humidity in an auto-controlled electric flue-curing oven to determine the changes of moisture content, pigments and main enzymes activities in different treatments. The results showed that the moisture content of different treatments varied greatly from 42 to 47℃. At the end of 42℃, CK > T1 > T2 > T3, while at the end of 47℃, CK > T2 > T3 > T1; chlorophyll content was higher in T1 from the beginning of curing to the end of 47℃, and carotenoid content was higher in T1 from the beginning of curing to the end of 42℃; the difference of carotenoid/chlorophyll ratio was the largest at the end of 47℃, showing T3 > T2 > CK > T1; amylase activity reached the first peak at the end of 38℃, and T3 > T2 > T1 > CK; polyphenol oxidase (PPO) activity was higher at CK from 38 to 54℃, and there was no significant difference between T1, T2 and T3; peroxidase (POD) activity reached the peak at the end of 38℃, CK, T2, T3 were close, and T1 was the lowest. In the process of flue-curing, the changes of moisture content, pigments and enzymes activities in different sections were roughly similar, but there were certain differences at different key stages of flue-curing, which provided a theoretical basis for optimizing flue-curing technology and improving the quality of flue-cured tobacco leaves.