Isolation and Diversity Analysis of Endophytic Bacteria in Jiyan 9
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摘要: 为了解烤烟内生细菌的多样性，以吉烟9号为供试材料，分别从汪清、和龙、延吉烤烟的根、茎、叶分离得到内生细菌24株、18株、16株，依据菌落表型特征及16S rRNA序列同源性对分离的内生菌进行初步鉴定和多样性分析，结果表明，主要为变形菌门（Proteobacteria）、放线菌门（Actinobacteria）、厚壁菌门（Firmicutes），其中的芽孢杆菌属（Bacillus）是3地共同的优势菌属。Neighbor-joining聚类分析表明，3个取样地烤烟内生细菌分别划分为13、9、11个类群。通过数值分类比较9个表型特征，结果表明，汪清、和龙在7.2水平聚为5个表观群，延吉在6.3水平聚为3个表观群。为吉烟9号内生菌的进一步应用提供了一定的理论基础。Abstract: To understand the diversity of endophytic bacteria in flue-cured tobacco, using Jiyan 9 of flue-cured tobacco as the test material, 24, 18 and 16 strains of endophytic bacteria were isolated from roots, stems and leaves of flue-cured tobacco from Wangqing, Helong and Yanji regions respectively. Preliminary identification and diversity analysis of isolated endophytes were carried out based on colony phenotypic characteristics and 16S rRNA sequence homology analysis. The results showed that the endophytes were mainly Proteobacteria, Actinobacteria, and Firmicutes. Among them, Bacillus is a common genus of the three locations. Neighbor-joining cluster analysis showed that the endophytic bacteria of the three sampled flue-cured tobaccos were divided into 13, 9, and 11 groups. Comparing 9 phenotypic features by numerical classification indicated that the Wangqing and Helong strains gathered at the 7.2 level for 5 apparent groups, and the Yanji strains was clustered into 3 apparent groups at the 6.3 level. It provides a theoretical basis for the further application of endophytic bacteria of Jiyan 9.
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