Abstract: Aiming at decreasing agricultural pollution caused by plastic films, degradation of photo-oxygen degradation and bio-degradable films and their effects on flue-cured tobacco development, soil temperature, moisture, enzyme activities and microbial community were studied and the results were compared with plastic film and open field. The results indicated that the degradation performance of bio-degradable film was better than photo-oxygen degradation film, with a mass loss rate of 59.9% after applied to flue-cured tobacco for 245 days. The development, biomass and compatibility of conventional chemical constituents of flue-cured tobacco increased markedly by film covering compared with open field, and the effects of plastic film was the best, followed by bio-degradable film, last for photo-oxygen degradation film. At the early stages of the tobacco plant growth, the temperature and moisture content of soil treated with two kinds of degradable film were higher than that of the open field, which was significantly lower than that of the plastic film. At the middle and later stages of the tobacco plant growth, it was similar to that of the plastic film. Film covering increased significantly the soil acid phosphatase activities and the OTUs of soil bacteria and fungi. In addition, the microbial diversity and evenness of soil fungi and soil bacteria were decreased and improved separately with the treatments of biodegradable film and photo-oxygen degradation film. Bio-degradable film had excellent degradability, and no significant difference in the development of flue-cured tobacco and soil enzyme activities, microbial diversity and evenness with plastic film, suggesting that it could be applied to tobacco production.