Abstract: Using Yunyan 87 as the test variety, the effects of combined application of wood vinegar and plant ash or mushroom residue on soil nutrients and growth of flue-cured tobacco were studied through pot experiments. Six treatments were included:clean water control (CK), single application of wood vinegar solution (T1), single application of grass ash (T2), combined application of wood vinegar solution + grass ash (T3), single application of mushroom residue (T4), combined application of wood vinegar solution + mushroom residue (T5). The results showed that there was no significant change in soil pH for tobacco planting with the single application of vinegar liquid, while the application of all the other modifiers increased soil pH. Compared with CK, all the treatment caused increase of soil organic matter, alkaline nitrogen, available phosphorus and effective potassium content, with more significant increase being observed in T3 and T5 treatments. At the same time, the combined application treatments were more conducive to improvement of agronomic characteristics and root structure of flue-cured tobacco, the accumulation of dry matter and tobacco pigment, and the promoting effect on root vigor of flue-cured tobacco was more significant. Compared with CK, root vigor of T3 and T5 treatments increased 66.02% and 68.45%, respectively. The combination of wood vinegar solution with mushroom residue or plant ash can improve soil nutrients of tobacco planting soil and promote growth of flue-cured tobacco, and the effect is better than that of single modifier application.