Screening for Bacterial Strains Promoting Seed Germination of Tobacco and Study of Their Growth-Promoting Characteristics
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摘要: 以芽孢杆菌属、贪铜菌属、假单胞菌属及土壤杆菌属的7株细菌菌株对烟草K326种子进行浸种处理，通过测定发芽率、发芽势、发芽指数及活力指数筛选到能够促进种子萌发的2株菌株；以钼蓝比色法测定培养液可溶磷含量，Salkowski法测定产IAA能力，CAS法测定分泌铁载体能力，对筛选出的2株菌的促生特性进行分析。结果表明，从贵州烟区土壤分离的2株高效氨化芽孢杆菌菌株可显著提高烟草种子的发芽指标，幼苗苗长显著高于未浸种处理。促生特性分析显示Bacillus toyonensis AMM-2和Bacillus mobilis AMM-5菌株的溶磷量分别为26.26和26.80 mg/L，产IAA的能力分别为21.57和7.40 μg/mL，铁载体分泌活性分别为35.08%和8.64%。研究显示，2株高效氨化细菌菌株具有优良的促生特性及显著的促种子萌发效应，可应用于烟草漂浮育苗，为促生有机肥的研制提供依据。Abstract: In order to screen for bacterial strains which promote seed germination, seeds of tobacco K326 were soaked with seven bacterial strains, which come from Bacillus sp., Cupriavidus sp., Pseudomonas sp. and Agrobacteria sp., and germination related indexes, including germination rate, germination potential, germination index and vigor index, were measured growth-promoting characteristics of two strains were determined. Soluble phosphorus contents were measured by molybdenum blue colorimetry. IAA contents and siderophore secreting capacity were measured by the methods of Salkowski and CAS, respectively. The results showed that germination related indexes of tobacco seeds were obviously increased after treated with two highly effective ammonifying Bacillus strains isolated from tobacco-planting soil in Guizhou. Bud length of these two treated groups was much higher than control. Phosphorus-dissolving contents of Bacillus toyonensis AMM-2 and Bacillus mobilis AMM-5 were 26.26 and 26.80 mg/L, IAA contents were 21.57 and 7.40 μg/mL, and siderosphere relative contents were 35.08% and 8.64%, respectively. So these two highly effective ammonifying Bacillus strains had good growth-promoting characteristics and significant effects on increasing seed germination of tobacco. They could be used for floating system and providing basis for developing growth-promoting organic fertilizers.
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