Abstract:
In order to determine the infection of TSWV in Shandong tobacco, specific primers were designed based on the existing TSWV genome sequence in GenBank. Through total RNA extraction, RT-PCR amplification, whole genome sequence determination and splicing analysis, the results showed that the suspected samples of Linyi, Shandong province were infected by TSWV. In order to further determine the whole genome sequence of the TSWV Linyi isolates, TSWV whole genome amplification primers were designed and synthesized. The results of cloning, sequencing and splicing analysis showed that the three RNA chains segments of the Shandong isolate are 8911, 4773 and 2971 bp in length, with more than 99.0% homology of nucleic acid sequence and 98.0% homology of protein sequence with the other reported Chinese isolates. Phylogenetic analysis showed that the Shandong isolate and the reported Yunnan TSWV isolate were clustered together in the same branch. It is speculated that TSWV in Shandong tobacco growing areas may have been introduced from Yunnan tobacco areas through the migration of toxic mediators. This study provides scientific evidence for the traceability of TSWV epidemics in Shandong tobacco growing areas and the accurate prediction and prevention of this dangerous viral disease.
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