Abstract: Tobacco bacterial wilt is a bacterial disease that seriously affects the production of tobacco in China. Screening for tobacco germplasm resistant to bacterial wilt and analyzing the genetic effects of resistance are of great significance for disease resistance breeding. In this study, the susceptible variety CB-1 and the bacterial wilt resistant mutant 153-K were selected as parents, and the F2 population was constructed. The analysis method of the mixed genetic model of "major gene + polygene" was used to study the genetic effects at two locations in Anhui and Fujian. The correlation analysis method was used to analyze the correlation between 153-K bacterial wilt resistance and agronomic traits. The results showed that the optimal genetic model of 153-K in Anhui is MX2-EEAD-AD, which means 2 equal dominant major genes + additive-dominant polygene model. The optimal genetic model in Fujian is MX2-ADI-AD, which means 2 pairs of additive-dominant-epistatic major gene + additive-dominant polygene model. The correlation analysis results showed that in Anhui and Fujian, resistance to bacterial wilt was significantly negatively correlated with plant height, but not significantly related with number of leaves, pitch distance, or stem circumference.