Abstract: A pot experiment was used to study the effects of four AM fungi (Fm, Ri, Ce, Gm) on the growth and mineral nutrient uptake of tobacco with different nitrogen efficiency ZY100 (low), K326 (medium), NC89 (high) with no inoculation (CK) as a control, which will clarify the effects of AM fungi on nutrient uptake of tobacco varieties with different nitrogen efficiency. The results indicated that four AM fungal strains could form a symbiotic relationship with roots of different tobacco genotypes. After inoculation with AM fungi, dry matter accumulation, plant height, maximum root length, root surface area, root volume, total root length, root tip number and nutrient uptake of each treated tobacco plant were increased to different degrees. The dry matter accumulationof each tobacco variety showed NC89>ZY100>K326, and the mycorrhiza dependence showed ZY100>NC89>K326. For mineral nutrients, after inoculated with AM fungi, the effects of increasing the accumulation of N, P, K, and Ca in each treatment were different with strain types and tobacco varieties, while the effects of increasing the accumulation of trace elements Fe, Mn, Cu and Zn were mainly affected by strain types, the accumulation of Mg was not significantly different among the treatments. It was shown that inoculation with AM fungi could effectively promote development of flue-cured tobacco root morphology, optimize root structure, and then promote uptake and accumulation of nutrients in tobacco plants. Overall, it is easier for ZY100 with low nitrogen efficiency to establish symbiotic relationship with AM strains, the Ce strain has a stronger affinity for the three varieties of flue-cured tobacco. In general, the Ce-ZY100 combination is the best for symbiotic establishment.