Abstract: In order to find out the difference in response to continuous cropping of three tobacco-planted soil types, taking red soil, paddy soil and purple soil in Yunnan Province as research objects, the changes of soil chemical indexes, enzyme activities and bacterial community characteristics under continuous cropping flue-cured tobacco for 5 years were analyzed. The results showed:after 5 years of continuous cropping, compared with the rotation treatment, the contents of organic matter, total nitrogen, total phosphorus, alkali hydrolyzable nitrogen and available phosphorus of paddy soil and red soil increased significantly, and the contents of pH value and available potassium decreased significantly, meanwhile only the content of available potassium of purple soil increased significantly, and other indexes did not change significantly. The catalase activity of the three soil types decreased significantly, the urease activity increased in varying degrees, but the increase of purple soil was not significant. The sucrase activity of paddy soil decreased significantly, and the change of red soil and purple soil were not obvious. The dominant flora of the three soil types were proteobacteria, cyanobacteria, acidobacteria, chloroflexi and bacteroidetes. After 5 years of continuous cropping, there were some differences in the relative abundance and activity of bacterial communities of different soil types. The structural composition of bacterial communities in purple soil was the most similar to that in the rotation treatment, and the changes of ACE and Shannon index were smaller in the three soil types. Comprehensive comparison showed that continuous cropping of flue-cured tobacco had little effect on the main biochemical indexes and microbial community structure of purple soil, indicating that purple soil was relatively insensitive to continuous cropping of flue-cured tobacco.