Abstract: The effects of different phosphate fertilizers on tobacco growth and soil phosphorus (P) forms were explored. Yunyan 87 and Niugantu soil were used as experimental materials in pot experiments. Six treatments of calcium superphosphate (T1), fused calcium-magnesium phosphate (T2), Ammonium polyphosphate (T3), Monoammonium phosphate (T4), Diammonium phosphate (T5) and no phosphorus (CK) were set for investigating and analysing the agronomic characteristics of tobacco, the Hedley grade phosphorus, and the correlation between them. The results showed that Resin-P, NaHCO₃-P_i, NaOH-P_i, HCl-P_i and residual-P were significantly increased, while organic phosphorus NaOH-P_o and NaHCO₃-P_o were significantly decreased in all P fertilizer treatments compared with no P application. The available phosphorus content of soil treated with fused calcium-magnesium phosphate and diammonium phosphate was significantly higher than that of other treatments, and the phosphorus activation coefficient of fused calcium-magnesium phosphate was the highest. Total fresh weight of tobacco leaves in each treatment was in the following order: T5>T2>T3>T1>T4>CK. Among them, all phosphorus treatments were significantly higher than the control. Each agronomic trait was positively correlated with Resin-P, NaHCO₃-P_i, total phosphorus, and available phosphorus, while most agronomic traits were negatively correlated with NaHCO₃-P_o and NaOH-P_o. In summary, fused calcium-magnesium phosphate and diammonium phosphate were highly effective in Niugantu soil, which was conducive to tobacco growth, leading to higher yield.