Study on Antioxidant Capacity and Energy Metabolism of Postharvest Tobacco Leaves with Different Maturity
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摘要: 为探究抗氧化能力和能量代谢在采后烟叶颜色变化中的作用,准确把握和理解采收成熟度,在自然条件下采用暗箱试验测定了不同成熟度采后烟叶的变黄程度、变褐程度、变黄指数、变褐指数、丙二醛(MDA)含量、浸出液电导率、抗氧化酶(SOD、POD、CAT)活性、能量相关物质(ATP、ADP和AMP)含量、能荷和呼吸代谢酶(CCO和SDH)活性。结果表明,随着采收成熟度的提高,采后烟叶变黄变褐发展更快,在试验期间的抗氧化酶(SOD、POD、CAT)和能量代谢酶(SDH、CCO)高活性时间持续更短。同时,高成熟度采后烟叶在试验中MDA含量和浸出液电导率更较高,且ATP含量高峰出现较早,ATP含量和能荷在试验期间保持高水平的持续时间更短。低成熟度采后烟叶变黄变褐发展更缓慢可能与其较高抗氧化能力和能量代谢有关。Abstract: for accurately grasping and understanding harvest maturity and explore the role of antioxidant capacity and energy metabolism in postharvest tobacco leaf discoloration, the harvested tobacco leaves were placed in a dark environment. The changes of yellowing, browning, MDA content, conductivity of leaching solution, adenosine triphosphate (ATP) content, adenosine diphosphate (ADP) content, adenosine triphosphate (AMP) content, energy charge (EG) and activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), succinate dehydrogenase (SDH) and cytochrome oxidase (CCO) of samples were determined during the experiment. With the increase of harvest maturity, the yellowing and browning of postharvest tobacco leaves started earlier and developed faster. During the test, the antioxidant enzymes (SOD, POD, CAT) and energy metabolism enzymes (SDH, CCO) were highly active. At the same time, the high-maturity postharvest tobacco leaves had higher MDA content and leachate conductivity in the experiment, and the ATP content peak appeared earlier, and the ATP content and energy charge remained high during the experiment for a shorter period of time. These results indicated that the slower development of yellowing and browning of tobacco leaves after harvest at low maturity may be related to higher antioxidant capacity and energy metabolism.
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[1] JIANG Y, JIANG Y, QU H, et al. Energy aspects in ripening and senescence of harvested horticultural crops[J]. Stewart Postharvest Review, 2007, 3(2): 1-5.
[2] LIU H, SONG L, YOU Y, et al. Cold storage duration affects litchi fruit quality, membrane permeability, enzyme activities and energy charge during shelf time at ambient temperature[J]. Postharvest Biology & Technology, 2011, 60(1): 24-30.
[3] 刘海. 高氧对香蕉采后呼吸代谢的影响[D]. 广州:华南师范大学,2003. LIU H. Effects of hyperoxia on postharvest respiration metabolism of bananas[D]. Guangzhou: South China Normal University, 2003. [4] 陈莲,陈梦茵,林河通,等. 解偶联剂DNP处理对采后龙眼果实果皮褐变和活性氧代谢的影响[J]. 中国农业科学,2009,42(11):4019-4026. CHEN L, CHEN M Y, LIN H T, et al. Effects of uncoupling agent DNP treatment on postharvest longan fruit peel browning and active oxygen metabolism[J]. China Agricultural Sciences, 2009, 42(11): 4019-4026.
[5] 宫长荣,李艳梅,李常军. 烘烤过程中烟叶脂氧合酶活性与膜脂过氧化的关系[J]. 中国烟草学报,2000(1):40-42. GONG C R, LI Y M, LI C J. The relationship between lipoxygenase activity and membrane lipid peroxidation in tobacco leaves during curing[J]. Acta Tobacco Sinica, 2000(1): 40-42.
[6] 黄山,杨虹琦,张发明,等. 烘烤温湿度变化对不同烤烟品种烟叶膜脂过氧化作用的影响[J]. 湖南农业大学学报(自然科学版),2009,35(5):485-492. HUANG S, YANG H G, ZHANG F M, et al. Effects of curing temperature and humidity changes on the membrane lipid peroxidation of different flue-cured tobacco varieties[J]. Journal of Hunan Agricultural University (Natural Science Edition), 2009, 35(5): 485-492.
[7] 韩锦峰,汪耀富,林学梧,等. 烤烟叶片成熟度与细胞膜脂过氧化及体内保护酶活性关系的研究[J]. 中国烟草学报,1994(1):20-24. HAN J F, WANG Y F, LIN X W, et al. Study on the relationship between flue-cured tobacco leaf maturity and cell membrane lipid peroxidation and protective enzyme activities in vivo[J]. Acta Tobacco Sinica, 1994(1): 20-24.
[8] 王传义,孙福山,王廷晓,等. 不同成熟度烟叶烘烤过程中生理生化变化研究[J]. 中国烟草科学,2009,30(3):49-53. WANG C Y, SUN F S, WANG T X, et al. Study on the physiological and biochemical changes of tobacco leaves with different maturity during the curing process[J]. China Tobacco Science, 2009, 30(3): 49-53.
[9] 李雪君,孙计平,丁燕芳,等. 烤烟新品种豫烟13号的选育及特征特性[J]. 中国烟草科学,2017,38(4):17-22. LI X J, SUN J P, DING Y F, et al. Breeding and characteristics of a new flue-cured tobacco variety "Yuyan 13[J]. China Tobacco Science, 2017, 38(4): 17-22.
[10] 陈颐,郑竹山,王建兵,等. 不同成熟度烤烟烘烤过程中游离氨基酸及转氨酶活性变化[J]. 中国烟草科学,2019,40(3):75-83. CHEN Y, ZHENG Z S, WANG J B, et al. Changes in free amino acids and transaminase activities of flue-cured tobacco with different maturity levels during curing[J]. China Tobacco Science, 2019, 40(3): 75-83.
[11] 国家烟草专卖局. 烤烟品种烘烤特性评价:YC/T 311—2009[S]. State Tobacco Monopoly Administration. Evaluation of curing characteristics of flue-cured tobacco varieties: YC/T 311—2009[S]. [12] 肖家欣,刘志文,罗充,等. 植物生理学实验[M] :合肥:安徽人民出版社,2010. XIAO J X, LIU Z W, LUO C, et al. Plant Physiology Experiment[M]: Hefei: Anhui Renmin Press, 2010. [13] YANG Q, ZHANG X, WANG F, et al. Effect of pressurized argon combined with controlled atmosphere on the postharvest quality and browning of sweet cherries[J]. Postharvest Biology and Technology, 2019, 147: 59-67.
[14] LIU H, JIANG Y, LUO Y, et al. A simple and rapid determination of ATP, ADP and AMP concentrations in pericarp tissue of litchi fruit by high performance liquid chromatography[J]. Food Technology and Biotechnology, 2006, 44(4): 531-534.
[15] IKUMA H, WALTER D. BONNER. Properties of higher plant mitochondria. I. Isolation and some characteristics of tightly-coupled mitochondria from dark-grown mung bean hypocotyls[J]. Plant Physiology, 1967, 42(1): 67-75.
[16] 陈京京,金鹏,李会会,等. 低温贮藏对桃果实冷害和能量水平的影响[J]. 农业工程学报,2012,28(4):275-281. CHEN J J, JIN P, LI H H, et al. Effects of low temperature storage on chilling injury and energy levels of peach fruits[J]. Transactions of the Chinese Society of Agricultural Engineering, 2012, 28(4): 275-281.
[17] KAN J, WANG H M, JIN C, et al. Changes of reactive oxygen species and related enzymes in mitochondrial respiration during storage of harvested peach fruits[J]. Agricultural Sciences in China, 2011, 10(1): 149-158.
[18] 王传义,张忠锋,徐秀红,等. 烟叶烘烤特性研究进展[J]. 中国烟草科学,2009,30(1):38-41. WANG C Y, ZHANG Z F, XU X H, et al. Research progress on curing characteristics of tobacco leaves[J]. China Tobacco Science, 2009, 30(1): 38-41.
[19] 张玉琴,李青山,王传义,等. 烤烟烟叶成熟过程中的颜色参数与烘烤特性研究[J]. 西南农业学报,2018,31(1):62-67. ZHANG Y Q, LI Q S, WANG C Y, et al. Study on color parameters and curing characteristics of flue-cured tobacco leaves during maturation[J]. Journal of Southwest Agriculture, 2018, 31(1): 62-67.
[20] 戴勋,王毅,刘彦中,等. 不同施氮量下云烟85不同成熟度烟叶的烘烤特性[J]. 湖北农业科学,2007,391(4):552-555. DAI X, WANG Y, LIU Y Z, et al. The curing characteristics of Yunyan 85 tobacco leaves with different maturity levels under different nitrogen application rates[J]. Hubei Agricultural Sciences, 2007, 391(4): 552-555.
[21] 武圣江,莫静静,娄元菲,等. 不同烤烟品种不同成熟度上部叶烘烤特性研究[J]. 核农学报,2020,34(6):1337-1349. WU S J, MO J J, LOU Y F, et al. Study on curing characteristics of upper leaves of different flue-cured tobacco varieties with different maturity[J]. Journal of Nuclear Agriculture, 2020, 34(6): 1337-1349.
[22] 王娟,李德全. 逆境条件下植物体内渗透调节物质的积累与活性氧代谢[J]. 植物学通报,2001(4):459-465. WANG J, LI D Q. Accumulation of osmotic adjustment substances and active oxygen metabolism in plants under adversity conditions[J]. Bulletin of Botany, 2001(4): 459-465.
[23] 肖茜,冯超,刘保华,等. 荔枝果皮褐变过程中膜脂过氧化及相关酶活性变化的比较研究[J]. 热带作物学报,2011,32(9):1653-1657. XIAO Q, FENG C, LIU B, et al. Study on the changes of membrane lipid peroxidation and related enzyme activities during the browning process of litchi pericarp [J]. Journal of Tropical Crops, 2011, 32(9): 1653-1657.
[24] 赵松超,李一凡,刘博远,等. 晾制密度对雪茄烟叶膜脂过氧化作用及品质的影响[J]. 作物学报,2019,45(7):1090-1098. ZHAO S C, LI Y F, LIU B Y, et al. Effects of drying density on membrane lipid peroxidation and quality of cigar tobacco leaves[J]. Acta Agronomica Sinica, 2019, 45(7): 1090-1098.
[25] 柳强. 红花大金元不同烟叶成熟度主要生化指标变化规律研究[D]. 贵阳:贵州大学,2010. LIU Q. Study on the change law of main biochemical indexes of different tobacco leaf maturity in “Honghuadayinyuan”[D], Guiyang: Guizhou University, 2010. [26] WANG F, YANG Q, ZHAO Q, et al. Roles of antioxidant capacity and energy metabolism in the maturity-dependent chilling tolerance of postharvest kiwifruit[J]. Postharvest Biology and Technology, 2020, 168: 111281.
[27] YI C, QU HX, JIANG Y, et al. ATP-induced changes in energy status and membrane integrity of harvested litchi fruit and its relation to pathogen resistance[J]. Journal of Phytopathology, 2008, 156(6): 365-371.
[28] 徐云岭,余叔文,植物适应盐逆境过程中的能量消耗[J]. 植物生理学通讯,1990(6):70-73. XU Y L, YU S W. Energy consumption in the process of plants adapting to salt stress[J]. Bulletin of Plant Physiology, 1990(6): 70-73.
[29] 赵云罡,徐建兴. 线粒体,活性氧和细胞凋亡[J]. 生物化学与生物物理进展,2001(2):168-171. ZHAO Y G, XU J X. Mitochondria, reactive oxygen species and apoptosis[J]. Progress in Biochemistry and Biophysics, 2001(2): 168-171.
[30] 潘俨. 库尔勒香梨果实发育及采后糖代谢与呼吸代谢关系的研究[D]. 乌鲁木齐:新疆农业大学,2016. PAN Y. The relationship between sugar metabolism and respiratory metabolism throughout fruit development and postharvest of Korla fragrant pear (Pyrus sinkiangensis Yu)[D]. Urumqi: Xinjiang Agricultural University, 2016. [31] ZHAO Z, CAO J, JIANG W, et al. Maturity-related chilling tolerance in mango fruit and the antioxidant capacity involved[J]. Journal of the Science of Food & Agriculture, 2010, 89(2): 304-309.
[32] PAN Y G, YUAN M Q, ZHANG W M, et al. Effect of low temperatures on chilling injury in relation to energy status in papaya fruit during storage[J]. Postharvest Biology and Technology, 2017, 125: 181-187.
[33] PENG J, HONG Z, LEI W, et al. Oxalic acid alleviates chilling injury in peach fruit by regulating energy metabolism and fatty acid contents[J]. Food Chemistry, 2014, 161: 87-93.
[34] CHENG S, WEI B, ZHOU Q, et al. 1-methylcyclopropene alleviates chilling injury by regulating energy metabolism and fatty acid content in ‘nanguo’ pears[J]. Postharvest Biology & Technology, 2015, 109: 130-136.
[35] 孙聪,白杨,李连国,等. 草莓果实线粒体呼吸代谢与超微弱发光的关系[J]. 西北植物学报,2019,39(10):1805-1811 . SUN C, BAI Y, LI L G, et al. The relationship between mitochondrial respiration metabolism and ultra-weak luminescence in strawberry fruit[J]. Journal of Northwest Botany, 2019, 39(10): 1805-1811
[36] 林安贵,杨灵灵. 植物提取物槲皮素调节小鼠的能量代谢和氧化应激[J]. 基因组学与应用生物学,2020,39(1):320-325. LIN A G, YANG L L. Plant extract quercetin regulates energy metabolism and oxidative stress in mice[J]. Genomics and Applied Biology, 2020, 39(1): 320-325.
[37] JIN P, ZHU H, WANG J, et al. Effect of methyl jasmonate on energy metabolism in peach fruit during chilling stress[J]. Journal of the Science of Food and Agriculture, 2013, 93(8): 1827-1832.
[38] LI C, CHENG Y, HOU J, et al. Application of methyl jasmonate postharvest maintains the quality of Nanguo pears by regulating mitochondrial energy metabolism[J]. Journal of Integrative Agriculture, 2021, 120(11): 3075-3083.
[39] 邓云龙,崔国民,孔光辉,等. 品种、部位和成熟度对烟叶淀粉含量及评吸质量的影响[J]. 中国烟草科学,2006(4):18-23 . DENG Y, CUI G M, KONG G H, et al. Effects of variety, location and maturity on starch content and smoking quality of tobacco leaves[J]. China Tobacco Science, 2006(4): 18-23
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