Abstract: In order to explore the feasibility of using near-infrared spectroscopy to evaluate the quality of tobacco strips quickly and comprehensively, flue-cured tobacco and tobacco strips from 2015 to 2017 were used as research materials. Prediction models of routine chemical compositions, aroma type, position and sensory quality were established by the partial least square method. The quality of Yunnan tobacco strips in 2018-2019 was used for comparative evaluation after verifying the effect of the model. The results showed that compared with 2018, the total sugar and reducing sugar decreased significantly in 2019, while total nitrogen increased significantly; The fresh aroma index decreased slightly. There was no significant difference in the index of upper, middle and lower positions between the two years. 57 of the 70 tobacco strips evaluated by the near-infrared spectroscopy sensory quality model were consistent with sensory evaluation, with a prediction accuracy of 81.4% and highly significant differences between years. The near-infrared spectroscopy prediction results were in high agreement with the sensory evaluation results. The use of near-infrared spectroscopy to evaluate the comprehensive quality of tobacco strips can provide a reference for cigarette formulation design.