Identification of Major-effect QTLs Associated with Leaf Width of Tobacco and Breeding Evaluation
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摘要: 为研究烟草叶宽性状调控的遗传规律，定位其主效QTL位点，利用烟草SNP芯片对构建的重组自交系（Recombinant Inbred Lines, RILs）群体（小黄金1025×Beinhart1000-1）进行基因分型，并使用IciMapping 4.2软件的ICIM-ADD方法在全基因组范围内定位到6个与叶宽性状相关的QTL，分别位于2、4、9、13、17和20号连锁群上，可以解释2.9%~36.8%的表型遗传变异；其中qMLW20-1可解释36.8%的表型变异，为主效基因。以K326为轮回亲本、Samsun为主效基因qMLW20-1供体亲本构建染色体片段代换系评估烟草叶宽主效QTL的遗传效应，结果表明，导入qMLW20-1主效基因能显著提高烟叶叶宽，其中G3材料显著提高了烟叶钾含量，感官质量等其他重要性状与对照无显著性差异，未携带不良性状基因，具有较好的育种利用价值。研究结果为克隆叶宽性状主效基因和进行分子改良奠定了基础。Abstract: In order to study the genetic regulation of leaf width traits in tobacco, to locate the dominant QTL loci and promote the breeding of tobacco varieties, the recombinant inbred lines (RILs) population (Xiaohuangjin 1025×Beinhart1000-1) was genotyped using a tobacco SNP chip. The ICIM-ADD method of IciMapping 4.2 software was used to identify six QTLs related to leaf width traits in the whole genome, which were located in groups 2, 4, 9, 13, 17 and 20, respectively and could explain 2.9% to 36.8% of the phenotypic genetic variation. qMLW20-1 accounted for 36.8% of the phenotypic variation and was the major-effect QTL. A chromosome fragment substitution line which using K326 as the transmutation parent and Samsun as the dominant gene qMLW20-1 donor parent was constructed to evaluate the genetic effect of the dominant gene QTL for leaf width in tobacco. The results showed that the introduction of qMLW20-1 as the dominant gene could significantly increase leaf width, and G3 significantly increased the potassium content in tobacco leaves. The sensory quality and other important characters had no significant difference from the control, and no bad character genes were carried. The line has good breeding utilization value. The results laid a foundation for cloning and molecular improvement of the major gene of leaf width trait.
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