Abstract: The spatial-temporal variation characteristics of soil available nutrients were studied to provide scientific basis for fertilization management of high yield and high quality in tobacco fields. The spatio-temporal evolution characteristics of soil available nitrogen, available phosphorus and available potassium in two typical counties of Xiangyun and Midu in Dali prefecture in 1982, 2012 and 2022 were analyzed by using geostatistics. From 1982 to 2022, the average content of soil available nitrogen in Xiangyun county increased first and then decreased. There was no significant difference in soil available nitrogen between three years in Midu, which was mainly grade III (100-150 mg/kg) and evenly distributed spatially. The contents of soil available phosphorus and available potassium in tobacco-growing areas showed an upward trend to various degrees, which were high in the north of Midu and the central and western parts of Xiangyun, and low in other regions. In recent 40 years, the nugget effect of soil available nutrients increased from <25% to 50%, indicating that the influence of natural factors such as topography on the spatial variation of nutrients was weakened, and human factors such as fertilization was enhanced. The content of soil available nitrogen was at a medium level (100-150 mg/kg), and the contents of available phosphorus and available potassium increased continuously due to the application of phosphorus and potassium fertilizers. According to the abundance and deficiency of soil available nutrients, for the tobacco fields in north central and west of Xiangyun and north of Midu we should 'stabilize nitrogen and reduce phosphorus and potassium', and for the rest areas we need to pay attention to 'stabilize nitrogen and control phosphorus and potassium'.