Abstract: In order to explore the effect of glycerin on the release of aroma components in different parts of flue-cured tobacco under low temperature heating (350 ℃), a programmed temperature-controlling coupled flue gas capture device was used to study the effects of different glycerin contents (0%, 5%, 10%, 15%, 20%, 25%, 30%) on the release of aroma components in upper, middle and lower flue-cured tobacco leaves. The results showed that: With the increase of glycerin content, the total amount of aroma components and nicotine release of tobacco leaves under heating conditions increased first and then slightly decreased, indicating that the addition of glycerin had a certain promoting effect on the release of aroma components. The total amount of various aroma components released from leaves from different positions the tobacco plant showed different increases with increasing glycerin content (0% to 15%): the total release of neutral aroma components in lower leaves increased most significantly by 95.11%, and the total release of alkaline and acidic aroma components in upper leaves increased most significantly, increasing 36.82% and 43.92%, respectively. The multivariate analysis of variance showed that glycerin content and leaf position had a very significant effect on the total release of various aroma components in tobacco leaves, and the interaction effect between leaf position and glycerin content was also extremely significant. Above all, the release of aroma components in tobacco leaves under low temperature heating could be promoted by applying 15% glycerin.