Abstract: To study the variation of quality characteristics of cigar tobacco leaves at different leaf positions and improve the industrial usability of tobacco leaves, we systematically analyzed the quality grade, appearance quality, and chemical composition differences of cigar tobacco leaves at each leaf position from six cigar tobacco varieties. The results indicated that the color changed from yellow brown-light brown-brown-reddish-brown with the rise of growing positions of cigar tobacco leaves. The vein, thickness, oil content and luster increased gradually. From bottom to top, the contents of nicotine, total nitrogen, total sugar, reducing sugar and chlorine in tobacco leaves increased gradually, while the contents of potassium and magnesium in tobacco leaves decreased gradually. There was a significant positive correlation between nicotine, total nitrogen, total sugar and reducing sugar in cigar tobacco leaves and six appearance quality indexes, while there was a significant negative correlation between potassium and magnesium and appearance quality. The cigar tobacco leaves at different leaf positions could be divided into four groups with different quality, namely P1~P3, P4~P10, P11~P14 and P15~P16. Among them, P1~P3, P15~P16 mainly produce filler tobacco leaves, P4~P10 mainly produce wrapper and binder, and most of the whole plant tobacco leaves are produced in this leaf group. P11~14 mainly produce binder and can produce a small amount of wrapper.