Abstract: Superior variety is basic for tobacco economic promotion. Since 2010, the rapid development of tobacco genomics led to the progress of tobacco genetic evolution, functional genetics, and provided new tools and approaches for tobacco breeding. We summarized the latest achievements in tobacco genome assembly and functional gene, described the current development of tobacco molecular marker-assisted selective breeding (MAS) involving construction of tobacco genetic linkage map, quantitative trait locus (QTL) localization, genome-wide association analysis (GWAS), and the joint analysis of QTL localization and GWAS. We also reviewed the technical theories of genome-wide selection (GS) and gene editing (GE) and their application progress in tobacco breeding. To improve the tobacco genome-assisted breeding (GAB), it is necessary to make full use of the information of tobacco genome, accelerate the development of molecular markers, promote the application of gene chips, and strengthen the construction of high-throughput phenotype monitoring platforms, so as to promote the application of GAB in tobacco breeding.