Abstract:
To assess the effect of
NtJAZ15 on tobacco glandular trichome development, the molecular mechanism underlying its involvement in trichome development was investigated. The
NtJAZ15 gene was cloned from K326, and its bioinformatics analysis and tissue expression patterns were examined. By generating
NtJAZ15 knockout and overexpression mutants, the regulatory roles of
NtJAZ15 in glandular trichome formationwere analyzed. Results indicated that both
NtJAZ15-1 and
NtJAZ15-2 were highly conserved across different tobacco varieties, exhibiting typical TIFY and Jas domains characteristic of the JAZ family.
NtJAZ15 was found to be expressed in all tissues, with
NtJAZ15-2 exhibiting higher expression levels compared to
NtJAZ15-1 in all tissues. Interestingly, the density of long and short glandular trichomes increased in
NtJAZ15 knockout mutants, while the opposite effect was observed in overexpressed mutants. Furthermore, qRT-PCR analysis showed that
NtJAZ15 significantly affected the expression of
NtWo,
NtCycB2,
NtHD9 and
NtHD12, but had no significant effect on the expression of
NtMIXTA. These findings together suggest that
NtJAZ15 negatively regulates the occurrence of long and short glandular trichome , and
NtWo,
NtCycB2,
NtHD9 and
NtHD12 may participate in the regulatory network involving
NtJAZ15.