Abstract: To screen efficient and safe herbicides against major harmful weeds in tobacco fields, indoor toxicity and field effect of 16 common pre-/post-seedling herbicides were detected on the gramineous weeds, broad-leaved species and sedge family by the tests of seed germination, indoor toxicity, and field control. The results showed that the inhibition rates of oxyfluorfen-metolachlor-pendimethalin and S-metolachlor ranged 45.7%-73.5% on seed germination of the tested weeds, yet with serious phytotoxicity to tobacco. S-metolachlor, napropamide, metolachlor, and clomazone-metolachlor were recommended to use before seedling emergence, with high toxicity to grass weeds while no harm to tobacco, and the field fresh mass control effect reaching 83.20%-92.57%. The post-seedling herbicides rimsulfuron-quizalofop-P-ethyl, rimsulfuron-haloxyfop-P-methyl, and rimsulfuron-clethodim, ranged their ED₅₀ for gramineous weeds as 56.0-264.6, 65.6-143.1, and 105.8-241.6 g/hm², respectively, while for broad-leaved as 172.7-303.8, 22.5-209.1, and 143.9-267.8 g/hm², respectively. They also exhibited field fresh mass control effects of 86.73%-92.82%, indicating better weed control effect and no harm, thus were recommended for the control of grass and broad-leaved weeds in tobacco fields. The research might lay a foundation for the precise control of weeds and rational use of herbicides in tobacco fields.