Abstract: Abstract: The relationships of chemical composition in tobacco leaf at the different altitudes and soil types of Luoping were studied in this paper. The results showed that, with the increase of altitudes, total sugar, reducing sugar, sugar-nicotine ratio, nitrogen-nicotine ratio and sulfur of tobacco leaf increased, and nicotine and total volatile alkali of tobacco leaf decreased. For red soil, there were significantly negative correlations between total sugar of tobacco leaf and available magnesium of soils, reducing sugar of tobacco leaf and available magnesium of soils. For yellow soil, there were significantly positive correlations between total sugar of tobacco leaf and available boron of soils, reducing sugar of tobacco leaf and available boron of soils. For alluvial soil, there were significantly positive correlations between reducing sugar of tobacco leaf and available boron of soils, sugar-nicotine ratio of tobacco leaf and available boron of soils, there were significantly negative correlation between nicotine of tobacco leaf and available boron of soils, total volatile alkali of tobacco leaf and available boron of soils, difference between total sugar and reducing sugar of tobacco leaf and effective boron of soils. In conclusion, chemical composition of tobacco leaf is the best from 1 600 to1 800 m altitudes and the condition of yellow soils.