Abstract: Spatial distribution of soil available micro-elements in tobacco planting fields was studied to provide theoretical basis for precision application of micro-fertilizers using geostatistics and GIS technique on small scale. Results showed that soil available microelements (Fe, Mn, Cu, and Zn) had medium variability with coefficient of variation from 22.0% to 48.4%. The semivariograms of available microelements could be fitted well with the spherical models. Kriging method was used to generate spatial distribution maps of soil available microelements, which indicated the content of soil available Cu and Mn had medium to high levels, while the 78% and 43% area of the whole study region were lack of soil available Fe and Zn, respectively.