Abstract: The cultural characteristics of race 0 of Phytophthora parasitica var. nicotianae were investigated in order to provide more basic information for further study and integrated management of the disease. The study used several cultural factors, including experimental medium, light, bacteria age, inducing agents and induction time to observe cultural characteristics of race 0 of Phytophthora parasitica var. nicotianae, such as growth rate of tobacco, sporangium production and tour dynamic spores release etc. The results showed that Oatmeal medium was suitable for mycelium growth and production of sporangium; light could limit its growth; based on the experimental period and other factors, mycelium cultivated for 21 days is more appropriate for induced; 0.1% KNO3 solution soaking mycelium contribute to sporangium production; after 72 h cultivation by 26℃, sudden drop of 12℃ for 0.5 h, combine with 1% glucose solution can promote sporangia to release zoospores, and it can also prolong time of zoospore's activities.