Abstract: The volatile aroma components from 390 leaf samples, including Honghua Dajinyuan, K326, Yunyan 87 and Yunyan 85 cultivars in Yunnan tobacco-growing areas were determined by SDE-GC/MS. The results showed that the contents of volatile aroma components, neoplytadiene and furans in upper and middle leaves from Honghua Dajinyuan and K326 cultivars were significantly higher than the ones from Yunyan 87 and Yunyan85 cultivars. The contents of alcohols and phenols in upper and middle leaves from Honghua Dajinyuan cultivar were significantly higher than the ones from K326, Yunyan 87 and Yunyan85 cultivars, but the contents of nitrogen-heterocyclic compounds were significantly lower than the latter.