Abstract: This study investigated the relationship between colorimetry indices and quality of tobacco leaves by using correlation analysis and stepwise regression analysis. The results showed that, the lightness of tobacco leaves had significant correlation with total nitrogen, total plant alkaloid, ratio of sugar to nicotine and ratio of nitrogen to nicotine, and was affected remarkably by total plant alkaloid. The red degree, hue angle and total value of chromatism had significant correlation with total sugar, total nitrogen, total plant alkaloid, ratio of sugar to nicotine, ratio of nitrogen to nicotine, and were affected remarkably by total nitrogen, total plant alkaloid. Yellowness had significant correlation with reducing sugar and potassium. Saturation had significant correlation with total sugar, reducing sugar, total nitrogen, potassium and ratio of sugar to nicotine. The yellowness and saturation were greatly influenced by potassium, but they had no significant correlation with the indices of sensory quality. The lightness, red degree, hue angle and total value of chromatism were all related to aroma quantity, taste, irritation, offensive gas significantly, among the colorimetry indices, the lightness, red degree, hue angle could be more effective in reflecting the situation of sensory quality indices which included aroma quantity, taste and irritation.