Abstract: Quality difference of aging tobacco leaf by three section cutting was investigated in this study. Electron microscopy analysis indicated that the difference of tobacco leaf surface structure was significant and there was tighter structure on surface of tobacco leaf tip. Surface groups difference was detected by fourier transform infrared spectrometry and content of carboxyl group and nitrogen group was difference. For the purpose of enhancing spectra resolution, the difference the second-derivative spectra was selected for evaluating the difference of tobacco leaf and obvious difference was observed in region of 700-1 200 cm-1, which suggested that varieties of sugar content among the three samples. Furthermore, the analysis of chemical component, pigment and aroma constituents showed that the sugar content of tobacco leaf center was the highest, while corresponding total nitrogen and chlorophylls was the lowest. However, the content of aroma constituents of tobacco leaf base was the highest. The final results showed that the quality of leaf center was the best, the next was leaf tip and the last was leaf base.