Abstract: The morphology and structure of glandular hairs of flue-cured tobacco variety Yunyan85 leaves was observed with Environmental Scanning Electron Microscope (ESEM). The results showed that the young leaves were densely covered by vigorous glandular hairs (include long-stalked glandular trichomes, short-stalked glandular trichomes and branch-stalked glandular hairs). The glandular hairs consisted of a basal cell, one or a few stalk cells and multi-cellular head. The long-stalked glandular hairs were bigger and the most (more than 80%); the short-stalked glandular hairs were thick and short and less; a part of branch-stalked glandular hairs were tree structure (arborization), and maximum individual and minimum quantity. The density of glandular hairs of ripe leaves were considerably less than those of young leaves, the appearances appear mostly to be in senescence, the stalk shrunk or lodged, the head sunken or opened or felled off. But, a few glandular hairs were still developing on the leaves at the some time.