Abstract: A field experiment was carried out to investigate the correlations between the activity of soil acid phosphatase and soil fertility under the condition of different manuring. The results showed that burying green manure on the activity of soil acid phosphatase had aftereffect. On the basis of burying green manure, reducing amount of chemical fertilize could improve the activity of soil acid phosphatase. Soil acid phosphatase activity and turn green manure gage pressure has certain positive correlation, when applying 70% chemical fertilizer. The correlation between the activity of soil acid phosphatase and soil available phosphorus in different periods varied much, namely positive correlation before tobacco transplanting and negative correlation in early and middle time of tobacco growing. There were significantly positive correlation between biomass of tobacco leaf and tobacco plant in early and middle time of tobacco growing. Therefore, as an indicator of soil phosphorus-supplying capacity, the activity of soil acid phosphatase was more scientific than soil available phosphorus.