Abstract: The laboratory bioassay and field control efficacy of Spodoptera litura nucleopolyhedrovirus (SpliNPV) Chenzhou strain were preliminarily examined. The efficient artificial propagation method was used to feed the host larvae with virus suspension, the average mortality of the insects was 65.0%. The death peak of the pests was in 4-8 days after infecting the virus. The high temperature and humidity, and poor light could help this virus. Filed control efficacy of Chenzhou strain was 86.6% in laboratory, which was better than another commercial strain. The corrected control efficacy of this strain was 88.4% in the fields, which was higher than that of avermectin pesticide significantly. It was detected that the occlusion body (OB) concentration of the initial virus’s stock solution was 1.03 ×10¹¹ OBs/ ml, and it was a strong SpliNPV stain, as it showed an excellent efficacy to control the pest Spodoptera litura, there will be a good prospect of application and development of this SpliNPV strain.