云南烤烟HPLC 指纹图谱数据库的建立和规程研究

Establishment of HPLC Fingerprints Analysis Procedure for Flue-cured Tobacco in Yunnan

摘要: 选择合适的方法对烟叶质量进行科学合理的分类对于提高卷烟质量和生产技术水平具有重要意义。对云南省典型烤烟的HPLC 指纹图谱分析规程进行了研究。规程包括样品取样和处理、特征提取剂的选择、仪器条件和分析及数据库的建立等4 个关键过程。首次提出了烤烟指纹图谱分时数据库的概念和建立方法。所建立的指纹图谱技术规程可作为烟叶质量分类的新方法。

Abstract: Choosing an appropriate method to classify tobacco quality is of significance for improving the quality of tobacco product. This study established a standardization procedure of HPLC fingerprint analysis for Yunnan flue-cured tobacco. The technology included four main parts, i.e., the sampling and sample processing, the selection of feature extraction agents, instrumentation, and fingerprint database building. The study proposed the concept of time-sharing database of flue-cured tobacco fingerprints and its building method. The fingerprint technology could be applied for classification and controlling of the quality of tobacco.