Abstract: Residue analysis of 8 carbamate pesticides in tobacco by reversed-phase high performance liquid chromatography was assessed. Residues were first extracted with acetonitrile and water, filtrated with NH₂-SPE columns, and then determined by post-column derivatization with fluorescence detector. The proposed methods showed a good linearity between analytical signal in the range of 0.1-1.0 mg/L with linear correlation coefficients of 0.9951-0.9999 and limits of detection of 0.008-0.01 mg/kg. The average recoveries obtained from samples spiked with standards at concentration level 0.05, 0.1 and 0.5 mg/kg were in the range of 80.8%-110.6%, 84.4%-109.7%和81.6%-112.9%, respectively, with relative standard deviations of 3.2%-10.8%, 3.8%-11.8%和3.7%-8.5%. Asatisfactory result was obtained in the simultaneous determination of carbamate pesticide residues in tobacco.