Abstract: In order to provide references for improving tobacco grading level scientifically, the chemical composition and polyphenol content in different grades of Honghuadajinyuan had been measured in this experiment. The differences and correlation of polyphenol and main chemical components had also been analyzed. The results showed that, with the increase of flue-cured tobacco leaves positions, polyphenol and nicotine content increased; the total sugar and reducing sugar content was the highest in middle leaves and the lowest in lower leaves. The polyphenol, total sugar and reducing sugar content in the upper and lower leaves was reduced with the leaves deepened; but in the same position and color leaves, the polyphenol, total sugar and reducing sugar content was increased with the grades heighten; the aromatic values in middle leaves was the highest in all of the leaves; the highly significant positive correlation were observed between the sugar and polyphenol; highly significant or significant negative correlations were detected between nitrogen and polyphenol.