Abstract: The purpose of this study was to identify the relationship between stable isotope and chemical components in flue-cured tobacco leaves by statistical approach. The results showed that variation of δ¹³C stable isotope was low but the variation of δ¹⁵N stable isotope was high in the tobacco leaves. The δ¹³C stable isotope was positively and significantly correlated with δ¹⁵N, positively and high significantly correlated with leaf total nitrogen content, negatively and significantly correlated with C/N and K⁺. The results from canonical correlation analysis showed that the potassium content and K/Cl in tobacco leaves decreased with the increasing of abundance of δ¹³C stable isotope, which demonstrated that there was possible relationship between stable isotope and chemical components, and chemical components in tobacco leaves could be influenced by the abundance of stable isotopes.