Abstract: In order to identify the weed species, their distribution and the noxious species in major tobacco-planting areas in Shiyan, Hubei province, and to provide technical support for high quality tobacco production, a survey was carried out in 8 towns in Yunxi, Zhushan and Fangxian, where tobacco-planting areas were the largest in Shiyan in September, 2011. All weed species and their numbers in a quadrat (50 cm × 50 cm) were recorded by using Diagonal Five-Point Sampling method. The results showed that there were 62 weed species, belonging to 27 families in tobacco fields in Shiyan. The dominant species were Digitaria sanguinalis, Echinochloa hispidula, Polygonum nepalense, Acalypha australis and Galinsoga parvifl. The weeds with high occurrence frequency were Digitaria sanguinalis, Acalypha australis, Polygonum nepalense, Siegesbeckia pubescens and Galinsoga parvifl. The altitude had some influence on weed species and their distribution. More weed species were found at lower altitudes than at higher altitudes. The weed species in tobacco fields in Shiyan was rich and their distribution was wide. Weed species varied in different altitudes and regions. According to weed density and frequency, the most noxious weed species in tobacco fields in Shiyan were Digitaria sanguinalis, Acalypha australis, and Polygonum nepalense, which should be paid much attention in weeds control.