Abstract: Tobacco-water relationship and the irrigation regulation are the research hot spot on tobacco water requirement as the theory basis for tobacco production and reasonable irrigation. This study summarized advance in tobacco-water relationship, including main theories and methods for estimating water requirement, influencing factors, water requirement characters in different growth stages, and water stress in tobacco. Finally, future research for tobacco-water relationship was proposed. More research at different ecotype under field condition should be made on the water requirement characters in tobacco, and new methods should be introduced to this area for increasing tobacco yield and quality.